Radioimmunoassay for leukotriene B4

Leukotriene B4 and asthma.

Murine Glomerular Leukotriene B4 Synthesis

Leukotriene (LT) B4 is an important pro-inflammatory autocoid. In order to investigate the potential role of this eicosanoid in renal inflammation, in this study we determined the capability of glomeruli to synthesize this mediator. Glomeruli were able to synthesize LTB4 when provided with exogenous substrate in a dose-dependent fashion in the presence of ionophore A23187. Ionophore, although b...

A Second Leukotriene B4 Receptor, Blt2

Leukotriene B(4) (LTB(4)) is a potent chemoattractant and activator of both granulocytes and macrophages. The actions of LTB(4) appear to be mediated by a specific G protein-coupled receptor (GPCR) BLT1, originally termed BLT (Yokomizo, T., T. Izumi, K. Chang, Y. Takuwa, and T. Shimizu. 1997. Nature. 387:620-624). Here, we report the molecular cloning of a novel GPCR for LTB(4), designated BLT2...

Urine leukotriene B4 in familial Mediterranean fever.

OBJECTIVE To determine urinary leukotriene B4 (LTB4) levels and their role in FMF: METHODS Urinary LTB4 levels were studied using a commercial ELISA kit in 12 FMF patients during abdominal attacks, and 20 FMF patients during remission. RESULTS Urinary LTB4 levels in FMF patients during attacks were comparable to those during remission, but higher than normal levels (p = 0.03). CONCLUSIONS...

Mechanisms of leukotriene B4--triggered monocyte adhesion.

OBJECTIVE Leukotriene B4 (LTB4) has been implicated in the trafficking of monocytes to inflammatory pathologic conditions, such as transplant rejection and atherosclerosis. The aim of this study was to determine the mechanisms by which LTB4 contributes to monocyte capture from the circulation. METHODS AND RESULTS In in vitro and in vivo vascular models, the lipid chemoattractant LTB4 was an e...